Home Research
Mentor/s:
Imilce A. Rodriguez Fernandez, PhD; Patricia V. Pujols, PhD
Project Title:
Evaluating different methods to identify changes in the gut microbiota – lessons from alcohol-exposed Drosophila melanogaster
Project Title:
This study compared Colony Forming Units (CFUs), Nanopore sequencing, and Biolog® EcoPlates to identify the most informative method for assessing gut microbiome changes in Drosophila melanogaster exposed to ethanol. CFUs and Nanopore sequencing detected alcohol-induced shifts in bacterial abundance and diversity, while EcoPlates failed to capture significant changes. Results showed increased Acetobacter spp. and age-dependent microbial community shifts, highlighting CFUs and Nanopore sequencing as reliable methods for studying alcohol’s impact on the gut microbiome.
Summer Research
Mentor/s:
Dustin E. Bosch, Bishal Parajuli, PhD
Project Title:
Using CRISPRi to study essential DNA repair genes in Bacteroides spp.
Project Title:
This study leverages CRISPR interference (CRISPRi) to inhibit essential DNA repair genes in Bacteroides thetaiotaomicron, a key gut commensal and potential pathogen. By targeting the RuvB protein involved in homologous recombination, the research aims to validate novel drug targets for selective antimicrobial strategies. Using inducible dCas9, RT-qPCR, and growth assays, the study assesses gene knockdown efficiency and bacterial sensitivity to DNA damage. Findings may inform microbiome-targeted therapies,offering alternatives to broad-spectrum antibiotics.
