{"id":18878,"date":"2024-06-10T13:37:04","date_gmt":"2024-06-10T13:37:04","guid":{"rendered":"https:\/\/natsci.uprrp.edu\/biology-new\/?page_id=18878"},"modified":"2025-11-14T07:58:33","modified_gmt":"2025-11-14T11:58:33","slug":"microscopy-facility","status":"publish","type":"page","link":"https:\/\/natsci.uprrp.edu\/biology\/microscopy-facility\/","title":{"rendered":"Microscopy Facility"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"18878\" class=\"elementor elementor-18878\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5dfc3e0c e-flex e-con-boxed e-con e-parent\" data-id=\"5dfc3e0c\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5cada1f5 elementor-widget elementor-widget-text-editor\" data-id=\"5cada1f5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t\n<p>Our&nbsp;<a href=\"http:\/\/www.cifupr.org\/\" target=\"_blank\" rel=\"noreferrer noopener\">Microscopy Facility<\/a>&nbsp;allows researchers to investigate cell ultrastructure using nondestructive imagining methods. The facility is equipped with a confocal and a two photon microscopes that allow studies of organelle structure and function and conjugated antibodies; measurements of DNA and RNA; identification of cytochemicals; determination of oxidative metabolism and andionic fluxes (Ca2+ flux); and surface imaging and 3D reconstruction, among others. We have a Zeiss LSM 510 confocal scan head that can be used with either our Zeiss Axiovert 100 inverted microscope or our Zeiss Axioplan 2 upright microscope. Confocal images can be acquired using either an Argon\/2 or He\/Ne visible light (458-633nM) lasers. A Coherent\u00ae Mira 900 infrared laser using a Coherent\u00ae Verdi V10 pump is used to acquire two photon microscopy images. This directly coupled, Ti:Sapphire laser can be tuned from 710nM to 980nM, thus giving us great flexibility when performing two photon microscopy studies.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Contact<\/h4>\n\n\n\n<p>Dr. Cesar Lasalde, Director<br>JGD 115<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Phone<\/h4>\n\n\n\n<p>(787) 76- 0000 x 1-2765<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Our&nbsp;Microscopy Facility&nbsp;allows researchers to investigate cell ultrastructure using nondestructive imagining methods. The facility is equipped with a confocal and a two photon microscopes that allow studies of organelle structure and function and conjugated antibodies; measurements of DNA and RNA; identification of cytochemicals; determination of oxidative metabolism and andionic fluxes (Ca2+ &hellip; <\/p>\n","protected":false},"author":4131,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-18878","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/pages\/18878","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/users\/4131"}],"replies":[{"embeddable":true,"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/comments?post=18878"}],"version-history":[{"count":3,"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/pages\/18878\/revisions"}],"predecessor-version":[{"id":21056,"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/pages\/18878\/revisions\/21056"}],"wp:attachment":[{"href":"https:\/\/natsci.uprrp.edu\/biology\/wp-json\/wp\/v2\/media?parent=18878"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}