Griselle Hernandez won the Best Poster Award Biosensors & Bioelectronics-2013
Graduate student Griselle Hernández-Cancel was awarded first price for her poster presentation at the 2nd International Conference and Exhibition on Biosensors & Bioelectronics; Chicago, IL.
The poster was titled: “Use of glycosylated horseradish peroxidase to improve the stability of an amperometric enzyme-based biosensor” and was authored by Griselle Hernández-Cancel, Damaris Suazo, Johnsue Medina-Guzmán, María Rosado-González, Liz Díaz, and Kai Griebenow.
In their poster the authors describe how they tackled a significant drawback of enzyme-based sensors, namely the rapid loss of activity because enzymes are very sensitive to their environment and thermodynamically intrinsically unstable. First, they developed a simple and low cost amperometric horseradish peroxidase (HRP) biosensor based on a 4-mercaptobenzoic acid self-assembled monolayer on gold nano-particles (AuNPs) at the surface of a glassy carbon (GC) electrode. The biosensors constructed employed chemically glycosylated and non-glycosylated HRP. The authors found that the chemically glycosylated form of the enzyme catalyzed the reduction of hydroquinone more rapidly and efficiently than the native form of the enzyme. In addition, glycosylated HRP showed an improvement in the biosensor sensitivity for detection of hydroquinone.
The detailed poster abstract can be found at the web site of the organizer at:http://www.omicsgroup.com/conferences/biosensors-bioelectronics-2013/posters.php